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cell apoptosis  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd cell apoptosis
    Elevated NETosis is observed in mice after challenged with CPA. (A–C) CPA intoxication induces an increase in NETs markers. (A) The levels of plasma MPO-DNA of PBS controls and CPA-treated at different time points were detected using ELISA. (B) The levels of plasma CitH3 of PBS controls and CPA-treated at different time points were detected using ELISA. (C) The levels of plasma cfDNA of PBS controls and CPA-treated at different time points were detected using the Picogreen method. Data are mean ± SD, n = 4. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. Control. ns, not significant). (D) Representative immunofluorescence images of CitH3 and MPO in neutrophils isolated from peripheral blood of PBS controls and CPA-treated. Scale bar = 10 μm. (E) Protein levels of PAD4 and CitH3 in peripheral blood, spleen and lung from PBS controls and CPA-treated were detected using Western blot ( n = 3). (F) Quantitative analysis of PAD4 and CitH3 in peripheral blood, spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (G) Representative images demonstrating NETs presence through co-expression of CitH3 and MPO in the spleen and lung subjected to CPA treated. Scale bar = 50 μm. (H) Quantitative analysis of MPO and CitH3 in spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (I) Caspase-3 activity was measured in neutrophils using a fluorometric assay. The relative Caspase-3 activity is shown, with minimal activation observed, indicating a lack of <t>apoptosis.</t> Flow cytometric analysis of neutrophils stained with Annexin V-PE and 7AAD. Representative dot plots show the distribution of cells in different quadrants: Q1 (Annexin V − /7AAD + ), Q2 (Annexin V + /7AAD + ), Q3 (Annexin V + /7AAD − ), and Q4 (Annexin V − /7AAD − ). The majority of neutrophils were found in Q4, indicating intact cell membranes and absence of phosphatidylserine exposure, which is inconsistent with apoptosis. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).
    Cell Apoptosis, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptosis+positive+control+solution/Apoptosis+Positive+Control+Solution/pmc12891852-172-0-10
    Average 98 stars, based on 2 article reviews
    cell apoptosis - by Bioz Stars, 2026-11
    98/100 stars

    Images

    1) Product Images from "Clostridium perfringens alpha toxin drives pathological NETosis via immature neutrophil mobilization and functional reprogramming"

    Article Title: Clostridium perfringens alpha toxin drives pathological NETosis via immature neutrophil mobilization and functional reprogramming

    Journal: Acta Pharmaceutica Sinica. B

    doi: 10.1016/j.apsb.2025.09.011

    Elevated NETosis is observed in mice after challenged with CPA. (A–C) CPA intoxication induces an increase in NETs markers. (A) The levels of plasma MPO-DNA of PBS controls and CPA-treated at different time points were detected using ELISA. (B) The levels of plasma CitH3 of PBS controls and CPA-treated at different time points were detected using ELISA. (C) The levels of plasma cfDNA of PBS controls and CPA-treated at different time points were detected using the Picogreen method. Data are mean ± SD, n = 4. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. Control. ns, not significant). (D) Representative immunofluorescence images of CitH3 and MPO in neutrophils isolated from peripheral blood of PBS controls and CPA-treated. Scale bar = 10 μm. (E) Protein levels of PAD4 and CitH3 in peripheral blood, spleen and lung from PBS controls and CPA-treated were detected using Western blot ( n = 3). (F) Quantitative analysis of PAD4 and CitH3 in peripheral blood, spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (G) Representative images demonstrating NETs presence through co-expression of CitH3 and MPO in the spleen and lung subjected to CPA treated. Scale bar = 50 μm. (H) Quantitative analysis of MPO and CitH3 in spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (I) Caspase-3 activity was measured in neutrophils using a fluorometric assay. The relative Caspase-3 activity is shown, with minimal activation observed, indicating a lack of apoptosis. Flow cytometric analysis of neutrophils stained with Annexin V-PE and 7AAD. Representative dot plots show the distribution of cells in different quadrants: Q1 (Annexin V − /7AAD + ), Q2 (Annexin V + /7AAD + ), Q3 (Annexin V + /7AAD − ), and Q4 (Annexin V − /7AAD − ). The majority of neutrophils were found in Q4, indicating intact cell membranes and absence of phosphatidylserine exposure, which is inconsistent with apoptosis. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).
    Figure Legend Snippet: Elevated NETosis is observed in mice after challenged with CPA. (A–C) CPA intoxication induces an increase in NETs markers. (A) The levels of plasma MPO-DNA of PBS controls and CPA-treated at different time points were detected using ELISA. (B) The levels of plasma CitH3 of PBS controls and CPA-treated at different time points were detected using ELISA. (C) The levels of plasma cfDNA of PBS controls and CPA-treated at different time points were detected using the Picogreen method. Data are mean ± SD, n = 4. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. Control. ns, not significant). (D) Representative immunofluorescence images of CitH3 and MPO in neutrophils isolated from peripheral blood of PBS controls and CPA-treated. Scale bar = 10 μm. (E) Protein levels of PAD4 and CitH3 in peripheral blood, spleen and lung from PBS controls and CPA-treated were detected using Western blot ( n = 3). (F) Quantitative analysis of PAD4 and CitH3 in peripheral blood, spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (G) Representative images demonstrating NETs presence through co-expression of CitH3 and MPO in the spleen and lung subjected to CPA treated. Scale bar = 50 μm. (H) Quantitative analysis of MPO and CitH3 in spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (I) Caspase-3 activity was measured in neutrophils using a fluorometric assay. The relative Caspase-3 activity is shown, with minimal activation observed, indicating a lack of apoptosis. Flow cytometric analysis of neutrophils stained with Annexin V-PE and 7AAD. Representative dot plots show the distribution of cells in different quadrants: Q1 (Annexin V − /7AAD + ), Q2 (Annexin V + /7AAD + ), Q3 (Annexin V + /7AAD − ), and Q4 (Annexin V − /7AAD − ). The majority of neutrophils were found in Q4, indicating intact cell membranes and absence of phosphatidylserine exposure, which is inconsistent with apoptosis. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).

    Techniques Used: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Immunofluorescence, Isolation, Western Blot, Two Tailed Test, Expressing, Activity Assay, Activation Assay, Staining

    CPA directly induces NETosis in both murine and human neutrophils in vitro . (A) Representative immunofluorescence images of purified neutrophils from mice stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (B) Representative immunofluorescence images of purified neutrophils from humans stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (C) Flow cytometric analysis of intracellular ROS production in mice PBMCs stimulated in vitro with PBS, CPA, or Rosup (positive control). Histograms show DCFH-DA fluorescence within the gated neutrophil population. (D) Flow cytometric analysis of intracellular ROS production in human PBMCs stimulated in vitro . Histograms show DCFH-DA fluorescence within the gated neutrophil population. (E) Relative mRNA expression of NETs-associated genes in stimulated mice PBMCs ( Tlr4 , Padi4 , Hmox1 , Nfe2l2 , and Cybb ) and human PBMCs ( TLR4 , PADI4 , HMOX1 , NFE2L2 , and CYBB ), as determined by qPCR. Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (F) Representative Western blot detecting PAD4 and CitH3 protein levels in lysates from stimulated mice PBMCs and human PBMCs ( n = 3). (G) Densitometric quantification of the protein bands shown in (F). Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (H) Caspase-3 activity measured by flow cytometry within the gated neutrophil population of stimulated mice PBMCs and human PBMCs. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test ( vs. PBS-treated. ns, not significant). (I) Flow cytometric analysis of apoptosis in stimulated mice PBMCs and human PBMCs using Annexin V and 7-AAD staining. Representative dot plots are shown. The bar graph shows the quantification of the live cell percentage (Annexin V − /7-AAD - ) within the gated neutrophil population. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).
    Figure Legend Snippet: CPA directly induces NETosis in both murine and human neutrophils in vitro . (A) Representative immunofluorescence images of purified neutrophils from mice stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (B) Representative immunofluorescence images of purified neutrophils from humans stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (C) Flow cytometric analysis of intracellular ROS production in mice PBMCs stimulated in vitro with PBS, CPA, or Rosup (positive control). Histograms show DCFH-DA fluorescence within the gated neutrophil population. (D) Flow cytometric analysis of intracellular ROS production in human PBMCs stimulated in vitro . Histograms show DCFH-DA fluorescence within the gated neutrophil population. (E) Relative mRNA expression of NETs-associated genes in stimulated mice PBMCs ( Tlr4 , Padi4 , Hmox1 , Nfe2l2 , and Cybb ) and human PBMCs ( TLR4 , PADI4 , HMOX1 , NFE2L2 , and CYBB ), as determined by qPCR. Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (F) Representative Western blot detecting PAD4 and CitH3 protein levels in lysates from stimulated mice PBMCs and human PBMCs ( n = 3). (G) Densitometric quantification of the protein bands shown in (F). Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (H) Caspase-3 activity measured by flow cytometry within the gated neutrophil population of stimulated mice PBMCs and human PBMCs. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test ( vs. PBS-treated. ns, not significant). (I) Flow cytometric analysis of apoptosis in stimulated mice PBMCs and human PBMCs using Annexin V and 7-AAD staining. Representative dot plots are shown. The bar graph shows the quantification of the live cell percentage (Annexin V − /7-AAD - ) within the gated neutrophil population. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).

    Techniques Used: In Vitro, Immunofluorescence, Purification, Positive Control, Staining, Fluorescence, Expressing, Western Blot, Activity Assay, Flow Cytometry, Two Tailed Test

    Related Articles

    Control:

    Article Title: ATP5A1 as a potential prognostic biomarker in clear-cell renal cell carcinoma
    Article Snippet: .. To the control cells, 500 μL of an Apoptosis Positive Control Solution (MultiSciences) was added, followed by incubation for 30 minutes. ..

    Article Title: Isolinderalactone regulates macrophage polarization and efferocytosis by activating the LXRα pathway against ulcerative colitis.
    Article Snippet: .. MODE-K cells were stained by CM-DIL and then induced by apoptosis-positive control solution (Multi Sciences, Hangzhou, China) on ice for 30 min according to the directions. ..

    Article Title: ATP5A1 as a potential prognostic biomarker in clear-cell renal cell carcinoma.
    Article Snippet: .. To the control cells, 500 μL of an Apoptosis Positive Control Solution (MultiSciences) was added, followed by incubation for 30 minutes. ..

    Article Title: Isolinderalactone regulates macrophage polarization and efferocytosis by activating the LXRα pathway against ulcerative colitis
    Article Snippet: .. MODE-K cells were stained by CM-DIL and then induced by apoptosis-positive control solution (Multi Sciences, Hangzhou, China) on ice for 30 min according to the directions. ..

    Positive Control:

    Article Title: ATP5A1 as a potential prognostic biomarker in clear-cell renal cell carcinoma
    Article Snippet: .. To the control cells, 500 μL of an Apoptosis Positive Control Solution (MultiSciences) was added, followed by incubation for 30 minutes. ..

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Article Title: Discovery of WWZ-11-098: a rigid and selective CDK6 degrader.
    Article Snippet: Dysregulation of the cell cycle, a hallmark of cancer, frequently involves aberrant activation of cyclin D-CDK4/6 complexes.. Although dual-CDK4/6 inhibitors are effective in advanced hormone receptor-positive (HR+), human epidermal growth factor receptor 2-negative (HER2-) breast cancer, CDK6 is preferentially expressed and functionally upregulated in hematologic malignancies, making it a promising therapeutic target.. The high structural homology between CDK4 and CDK6, however, has made developing selective CDK6 inhibitors exceptionally difficult.

    Article Title: ATP5A1 as a potential prognostic biomarker in clear-cell renal cell carcinoma.
    Article Snippet: .. To the control cells, 500 μL of an Apoptosis Positive Control Solution (MultiSciences) was added, followed by incubation for 30 minutes. ..

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer.
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Article Title: Ginsenoside Rg3 ameliorates acute pancreatitis by activating the NRF2/HO-1-mediated ferroptosis pathway
    Article Snippet: The AR42J cells (10 6 cells/well in a 6-well plate (Corning, Inc.) were pre-incubated with or without Rg3 for 1 h at 37°C and then treated with Cn (10 −8 M, cat. no. HY-A0190, MedChemExpress) for 24 h. Cell death was quantified using an Annexin V-PE/7-AAD apoptosis kit (cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.). .. Cells were washed with ice-cold PBS three times and resuspended in 500 μ l Apoptosis Positive Control Solution [cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.]. .. Following three washes with PBS, 1X binding buffer [cat. no. AP104-30; Multi Sciences (LIANKE) Biotech, Co., Ltd.] was added.

    Incubation:

    Article Title: ATP5A1 as a potential prognostic biomarker in clear-cell renal cell carcinoma
    Article Snippet: .. To the control cells, 500 μL of an Apoptosis Positive Control Solution (MultiSciences) was added, followed by incubation for 30 minutes. ..

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Article Title: Discovery of WWZ-11-098: a rigid and selective CDK6 degrader.
    Article Snippet: Dysregulation of the cell cycle, a hallmark of cancer, frequently involves aberrant activation of cyclin D-CDK4/6 complexes.. Although dual-CDK4/6 inhibitors are effective in advanced hormone receptor-positive (HR+), human epidermal growth factor receptor 2-negative (HER2-) breast cancer, CDK6 is preferentially expressed and functionally upregulated in hematologic malignancies, making it a promising therapeutic target.. The high structural homology between CDK4 and CDK6, however, has made developing selective CDK6 inhibitors exceptionally difficult.

    Article Title: ATP5A1 as a potential prognostic biomarker in clear-cell renal cell carcinoma.
    Article Snippet: .. To the control cells, 500 μL of an Apoptosis Positive Control Solution (MultiSciences) was added, followed by incubation for 30 minutes. ..

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer.
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Staining:

    Article Title: Isolinderalactone regulates macrophage polarization and efferocytosis by activating the LXRα pathway against ulcerative colitis.
    Article Snippet: .. MODE-K cells were stained by CM-DIL and then induced by apoptosis-positive control solution (Multi Sciences, Hangzhou, China) on ice for 30 min according to the directions. ..

    Article Title: Isolinderalactone regulates macrophage polarization and efferocytosis by activating the LXRα pathway against ulcerative colitis
    Article Snippet: .. MODE-K cells were stained by CM-DIL and then induced by apoptosis-positive control solution (Multi Sciences, Hangzhou, China) on ice for 30 min according to the directions. ..

    Centrifugation:

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer.
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Binding Assay:

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..

    Article Title: High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer.
    Article Snippet: .. The pellet was resuspended in 500 μl Apoptosis Positive Control Solution (Liankebio, China; Cat# AT107) and incubated on ice for 30 min. After centrifugation and PBS washing, cells were resuspended in 500 μl 1× Binding Buffer. ..



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    Multi Sciences (Lianke) Biotech Co Ltd apoptosis assay
    Combination of KPT-330 and oxaliplatin induces <t>apoptosis</t> in oxaliplatin-resistant colorectal cancer cells. (A) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h, assessed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (B) Viability of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed using a Cell Counting Kit-8 assay. (C) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (D) Immunoblot analysis of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h. ** P<0.01, *** P<0.001, **** P<0.0001. PARP, poly (ADP-ribose) polymerase.
    Apoptosis Assay, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptosis+positive+control+solution/Apoptosis+Positive+Control+Solution/pm41340134-128-7-16
    Average 98 stars, based on 1 article reviews
    apoptosis assay - by Bioz Stars, 2026-11
    98/100 stars
    		  Buy from Supplier
    apoptosis assays  (Multi Sciences (Lianke) Biotech Co Ltd)
    98
    Multi Sciences (Lianke) Biotech Co Ltd apoptosis assays
    Combination of KPT-330 and oxaliplatin induces <t>apoptosis</t> in oxaliplatin-resistant colorectal cancer cells. (A) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h, assessed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (B) Viability of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed using a Cell Counting Kit-8 assay. (C) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (D) Immunoblot analysis of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h. ** P<0.01, *** P<0.001, **** P<0.0001. PARP, poly (ADP-ribose) polymerase.
    Apoptosis Assays, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptosis+positive+control+solution/Apoptosis+Positive+Control+Solution/pm41319938-92-1-21
    Average 98 stars, based on 1 article reviews
    apoptosis assays - by Bioz Stars, 2026-11
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Elevated NETosis is observed in mice after challenged with CPA. (A–C) CPA intoxication induces an increase in NETs markers. (A) The levels of plasma MPO-DNA of PBS controls and CPA-treated at different time points were detected using ELISA. (B) The levels of plasma CitH3 of PBS controls and CPA-treated at different time points were detected using ELISA. (C) The levels of plasma cfDNA of PBS controls and CPA-treated at different time points were detected using the Picogreen method. Data are mean ± SD, n = 4. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. Control. ns, not significant). (D) Representative immunofluorescence images of CitH3 and MPO in neutrophils isolated from peripheral blood of PBS controls and CPA-treated. Scale bar = 10 μm. (E) Protein levels of PAD4 and CitH3 in peripheral blood, spleen and lung from PBS controls and CPA-treated were detected using Western blot ( n = 3). (F) Quantitative analysis of PAD4 and CitH3 in peripheral blood, spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (G) Representative images demonstrating NETs presence through co-expression of CitH3 and MPO in the spleen and lung subjected to CPA treated. Scale bar = 50 μm. (H) Quantitative analysis of MPO and CitH3 in spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (I) Caspase-3 activity was measured in neutrophils using a fluorometric assay. The relative Caspase-3 activity is shown, with minimal activation observed, indicating a lack of apoptosis. Flow cytometric analysis of neutrophils stained with Annexin V-PE and 7AAD. Representative dot plots show the distribution of cells in different quadrants: Q1 (Annexin V − /7AAD + ), Q2 (Annexin V + /7AAD + ), Q3 (Annexin V + /7AAD − ), and Q4 (Annexin V − /7AAD − ). The majority of neutrophils were found in Q4, indicating intact cell membranes and absence of phosphatidylserine exposure, which is inconsistent with apoptosis. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Clostridium perfringens alpha toxin drives pathological NETosis via immature neutrophil mobilization and functional reprogramming

    doi: 10.1016/j.apsb.2025.09.011

    Figure Lengend Snippet: Elevated NETosis is observed in mice after challenged with CPA. (A–C) CPA intoxication induces an increase in NETs markers. (A) The levels of plasma MPO-DNA of PBS controls and CPA-treated at different time points were detected using ELISA. (B) The levels of plasma CitH3 of PBS controls and CPA-treated at different time points were detected using ELISA. (C) The levels of plasma cfDNA of PBS controls and CPA-treated at different time points were detected using the Picogreen method. Data are mean ± SD, n = 4. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. Control. ns, not significant). (D) Representative immunofluorescence images of CitH3 and MPO in neutrophils isolated from peripheral blood of PBS controls and CPA-treated. Scale bar = 10 μm. (E) Protein levels of PAD4 and CitH3 in peripheral blood, spleen and lung from PBS controls and CPA-treated were detected using Western blot ( n = 3). (F) Quantitative analysis of PAD4 and CitH3 in peripheral blood, spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (G) Representative images demonstrating NETs presence through co-expression of CitH3 and MPO in the spleen and lung subjected to CPA treated. Scale bar = 50 μm. (H) Quantitative analysis of MPO and CitH3 in spleen and lung from mice PBS controls and CPA-treated. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (I) Caspase-3 activity was measured in neutrophils using a fluorometric assay. The relative Caspase-3 activity is shown, with minimal activation observed, indicating a lack of apoptosis. Flow cytometric analysis of neutrophils stained with Annexin V-PE and 7AAD. Representative dot plots show the distribution of cells in different quadrants: Q1 (Annexin V − /7AAD + ), Q2 (Annexin V + /7AAD + ), Q3 (Annexin V + /7AAD − ), and Q4 (Annexin V − /7AAD − ). The majority of neutrophils were found in Q4, indicating intact cell membranes and absence of phosphatidylserine exposure, which is inconsistent with apoptosis. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).

    Article Snippet: Cell apoptosis was measured using an Annexin V-PE/7AAD apoptosis kit (MULTISCIENCES, AT104-100, Hangzhou, China) and Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) (Cell Signaling Technology, 9669S, Danvers, MA, USA).

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Immunofluorescence, Isolation, Western Blot, Two Tailed Test, Expressing, Activity Assay, Activation Assay, Staining

    CPA directly induces NETosis in both murine and human neutrophils in vitro . (A) Representative immunofluorescence images of purified neutrophils from mice stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (B) Representative immunofluorescence images of purified neutrophils from humans stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (C) Flow cytometric analysis of intracellular ROS production in mice PBMCs stimulated in vitro with PBS, CPA, or Rosup (positive control). Histograms show DCFH-DA fluorescence within the gated neutrophil population. (D) Flow cytometric analysis of intracellular ROS production in human PBMCs stimulated in vitro . Histograms show DCFH-DA fluorescence within the gated neutrophil population. (E) Relative mRNA expression of NETs-associated genes in stimulated mice PBMCs ( Tlr4 , Padi4 , Hmox1 , Nfe2l2 , and Cybb ) and human PBMCs ( TLR4 , PADI4 , HMOX1 , NFE2L2 , and CYBB ), as determined by qPCR. Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (F) Representative Western blot detecting PAD4 and CitH3 protein levels in lysates from stimulated mice PBMCs and human PBMCs ( n = 3). (G) Densitometric quantification of the protein bands shown in (F). Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (H) Caspase-3 activity measured by flow cytometry within the gated neutrophil population of stimulated mice PBMCs and human PBMCs. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test ( vs. PBS-treated. ns, not significant). (I) Flow cytometric analysis of apoptosis in stimulated mice PBMCs and human PBMCs using Annexin V and 7-AAD staining. Representative dot plots are shown. The bar graph shows the quantification of the live cell percentage (Annexin V − /7-AAD - ) within the gated neutrophil population. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Clostridium perfringens alpha toxin drives pathological NETosis via immature neutrophil mobilization and functional reprogramming

    doi: 10.1016/j.apsb.2025.09.011

    Figure Lengend Snippet: CPA directly induces NETosis in both murine and human neutrophils in vitro . (A) Representative immunofluorescence images of purified neutrophils from mice stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (B) Representative immunofluorescence images of purified neutrophils from humans stimulated in vitro with PBS, CPA, or PMA (positive control). Cells were stained for CitH3 (red) and MPO (green). Scale bar = 10 μm. (C) Flow cytometric analysis of intracellular ROS production in mice PBMCs stimulated in vitro with PBS, CPA, or Rosup (positive control). Histograms show DCFH-DA fluorescence within the gated neutrophil population. (D) Flow cytometric analysis of intracellular ROS production in human PBMCs stimulated in vitro . Histograms show DCFH-DA fluorescence within the gated neutrophil population. (E) Relative mRNA expression of NETs-associated genes in stimulated mice PBMCs ( Tlr4 , Padi4 , Hmox1 , Nfe2l2 , and Cybb ) and human PBMCs ( TLR4 , PADI4 , HMOX1 , NFE2L2 , and CYBB ), as determined by qPCR. Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (F) Representative Western blot detecting PAD4 and CitH3 protein levels in lysates from stimulated mice PBMCs and human PBMCs ( n = 3). (G) Densitometric quantification of the protein bands shown in (F). Data are mean ± SD, n = 3. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test (∗ P < 0.05; ∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant). (H) Caspase-3 activity measured by flow cytometry within the gated neutrophil population of stimulated mice PBMCs and human PBMCs. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test ( vs. PBS-treated. ns, not significant). (I) Flow cytometric analysis of apoptosis in stimulated mice PBMCs and human PBMCs using Annexin V and 7-AAD staining. Representative dot plots are shown. The bar graph shows the quantification of the live cell percentage (Annexin V − /7-AAD - ) within the gated neutrophil population. Data are mean ± SD, n = 3. Statistical significance was determined using an unpaired two-tailed t- test (∗∗∗ P < 0.001 vs. PBS-treated. ns, not significant).

    Article Snippet: Cell apoptosis was measured using an Annexin V-PE/7AAD apoptosis kit (MULTISCIENCES, AT104-100, Hangzhou, China) and Cleaved Caspase-3 (Asp175) Antibody (Alexa Fluor® 488 Conjugate) (Cell Signaling Technology, 9669S, Danvers, MA, USA).

    Techniques: In Vitro, Immunofluorescence, Purification, Positive Control, Staining, Fluorescence, Expressing, Western Blot, Activity Assay, Flow Cytometry, Two Tailed Test

    Combination of KPT-330 and oxaliplatin induces apoptosis in oxaliplatin-resistant colorectal cancer cells. (A) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h, assessed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (B) Viability of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed using a Cell Counting Kit-8 assay. (C) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (D) Immunoblot analysis of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h. ** P<0.01, *** P<0.001, **** P<0.0001. PARP, poly (ADP-ribose) polymerase.

    Journal: International Journal of Molecular Medicine

    Article Title: Exportin 1 inhibitor KPT-330 reverses oxaliplatin resistance via p53 nuclear retention in colorectal cancer

    doi: 10.3892/ijmm.2025.5675

    Figure Lengend Snippet: Combination of KPT-330 and oxaliplatin induces apoptosis in oxaliplatin-resistant colorectal cancer cells. (A) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h, assessed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (B) Viability of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed using a Cell Counting Kit-8 assay. (C) Apoptosis rate of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin and KPT-330 or the combination with Z-VAD-FMK for 48 h, analyzed by flow cytometry. The sum of early apoptotic cells (annexin V + /PI - ) in the lower-right quadrant and late apoptotic cells (annexin V + /PI + ) in the upper-right quadrant indicates apoptotic cells, and was used for quantification. (D) Immunoblot analysis of HCT116/L-OHP and HCT8/L-OHP cells treated with oxaliplatin, KPT-330 or the combination for 48 h. ** P<0.01, *** P<0.001, **** P<0.0001. PARP, poly (ADP-ribose) polymerase.

    Article Snippet: Apoptosis was detected using the Annexin V-FITC/PI kit [cat. no. AT101C; MultiSciences (Lianke) Biotech Co., Ltd.].

    Techniques: Flow Cytometry, Cell Counting, Western Blot